Expression of odontogenic markers in stem cells from human exfoliated deciduous teeth treated with perivitelline fluid from horseshoe crab

Ibrahim N, Mohamad Nasir NF, Azmi NI, Kannan TP

Abstract


Stem cells from human exfoliated deciduous teeth (SHED) makes an ideal source for mesenchymal stem cells (MSCs) for cell-based regenerative therapies due to their ability to differentiate into wide range of cells lineages. Teeth regeneration requires regulated odontogenesis that will generate dentin. Previous studies have shown the potential of perivitelline fluid (PVF) to act as a supplement material in enhancing proliferation of cells and promoting the generation of certain organs due to its high primitive protein content. This study analysed the expression of odontogenesis markers in SHED treated with PVF (0.019 mg/mL) on day 1, 3, and 7. The expression of dentin sialophosphoprotein (DSPP), dentin matrix acidic phosphoprotein 1 (DMP-1), osteopontin (OPN) and runt-related transcription factor 2 (RUNX2) were analysed at the transcript level in two groups of SHED which are PVF treated group (0.019 mg/ml) and without PVF. All four genes expressed at higher level in treatment group as compared to control on all tested days. Significant difference in gene expression between the control and treated groups were found on all tested days except for RUNX2 gene whereby its expression was not significantly different at day 7. Thus, it is suggested that PVF has the potential to enhance odontogenesis in SHED.

Keywords


Deciduous teeth, gene expression, horseshoe crab, odontogenesis, perivitelline fluid, stem cells

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References


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